Multiplex mRNA Profiling (PrimeFlow)
Multiplex mRNA Profiling
PrimeFlow
PrimeFlow technology offers superior specificity, single cell protein and kinetic analysis, high throughput capability, and simultaneous monitoring of several target RNAs within the same cell type or distinct cell populations in a heterogeneous cell sample. This assay employs fluorescence in situ hybridization (FISH) with branched-DNA signal amplification for simultaneous detection of RNA targets. Further, RNA labeling can be combined with cell surface and intracellular fluorescent antibody staining which adds a greater degree of specificity.Our multiplex RNA assay reveals the dynamics of both RNA and protein expression within individual cells, facilitating unprecedented analysis of their correlation as the cell changes over time or in response to a stimulus.
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