Discovery Biology & Pharmacology Services

We are committed to support your success of drug discovery

In Vitro Discovery Biology

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  • Cytotoxicity and proliferation assays: 3D cell culture; cell cycle analysis; microtubule and mitosis test
  • Angiogenesis assays: endothelial cell (EC) adhesion, tube formation, aorta ring explant, VEGFR signaling
  • Epigenetics assays: Acetyltransferase, DNA Methyltransferase, HDAC, Histone Demethylase, Histone Methyltransferase, Bromodomain, PARP screening, and profiling
  • Live cell imaging: adhesion, migration, chemotaxis and wound healing
  • Cell-free assays: various kinase, phosphatase, and other enzymatic assays
  • Simultaneous multiplex analysis mRNA and proteins
  • Reporter gene assays
  • In-cell western blot (ICW) based compound screening assays
  • Traditional western blot, SDS-PAGE, and 2-D gel

Translational Development

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  • Biomarker validation and measurement using Simple Western
  • Characterization of mechanism of action of novel drugs
  • Screening of anti-aging agents for cosmetics
  • Development of companion diagnostic methods
  • Drug combination screening for synergistic studies
  • Analysis of post-translational modification of proteins
  • Target identification and validation of drug candidates and novel compounds
  • Genome-wide gene array, low-density gene array, and real-time RT-PCR

PROTAC Discovery

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  • Identify and characterize new, potent, and selective ligands that bind and reprogram E3 ligase substrate specificity with our ligand binding assay.
  • Develop, characterize, and validate the warhead end of novel PROTACs.
  • Enable sensitive quantitation of PROTAC-mediated degradation of targets for DC50 and Dmax values 
  • Evaluate the impact of target degraders on efficacy and safety-related translational biomarkers with our automatic western blot, IHC and other platforms.
  • Characterize PROTAC selectivity across the proteome and provide a global readout on changes in protein expression using protein microarray such as RPPA.
  • Provide on-shelf assays, such as CRBN, BET-VHL binding assay,   VHL and CRBN ternary complex formation assay

Multiplex Live Cell Imaging

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  •  Cell adhesion, migration, chemotaxis and wound healing

3-D In Vitro Models

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  • 3-D anti-psoriatic drug screening 
  • 3-D cancer cell viability assay  
  • 3-D spheroid cell invasion assay
  • 3-D tumor micro-tissue assay
  • 3-D anti-skin aging cosmetic ingredient screening

In vitro Toxicology/Safety Test

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  • Analysis of DNA damage (comet, DNA fiber assay), DNA oxidation; DNA methylation 
  • Ames test (bacterial reverse mutation, 2 or 5 strains
  • Micronucleus test (MNT)
  • Mitochondrial toxicity
  • CFU assay for hematotoxicity assessment
  • Colony-forming cell-fibroblast (CFU-F) assay for mesenchymal toxicity assessment
  • Cell-specific cytotoxicity