Reverse Phase Protein Array (RPPA)
RPPA is a high-throughput antibody-based micro-array with procedures similar to Western blots. Proteins are extracted from tissues or cells, denatured by SDS, printed on nitrocellulose-coated slides followed by antibody probes.
- Cost-effective and highly efficient method utilizing automation for increased quality and reliability
- Simple sample preparation
- Robust quantification due to serial dilution and sample replicates
- Characterization of cellular signaling networks under different conditions
- Determination of drug selectivity and identification of therapeutic targets
- Definition of regulatory mechanisms in signaling networks, including cross-talk and feedback loops
- Classification of patient tumors, prognosis determination, and response predictions to targeted therapies
- Understanding of pharmaco-dynamics and biologically relevant dosing and scheduling
Digi-Western Array
The Digi-Western Array is a high-throughput antibody-based microarray that combines SDS-PAGE based protein separation with immobilization on Luminex beads. The combination of MW resolution, sensitivity, and signal linearity on the Luminex platform enables rapid quantification of hundreds of specific proteins and protein modifications in complex samples. This array is able to parallel profile up to 600 total phosphoproteins in only 50 ug of samples (cells, tissues, etc.) to support biomarker discovery pathway mapping, drug MoA studies, compound characterization, etc.
