Luminex Multiplex Immunoassay Service

Luminex multiplex immunoassay service

Luminex assays are high-throughput, multiplex bead-based assays. We are able to simultaneously examine multiple analytes (protein and mRNA) in a single sample (as little as 10 μL per sample from human, mouse, etc.). This includes ~150 mouse cytokines/chemokines, ~300 human cytokines/chemokines, and many proteins (expression or phosphorylation level) involved in the signaling pathways of cancer, apoptosis, acute phase response, inflammation, immunity, diabetes, kidney toxicity, and more. We can also simultaneously investigate multiple histone H3 PTMS, transcription factors-specific DNA binding activity, gene expression, and DNA copy number variation.

Luminex allows:

  • Quantitation and detection of cytokines / chemokine, cancer biomarkers or signal transduction proteins in cell lysates, cell culture supernatants, serum, plasma, etc.
  • Simultaneously detect and quantify multiple analytes (proteins, peptides, or nucleic acids) in a single sample volume with superior performance and reproducible results
  • Simultaneously interrogate multiple protein phosphorylation and histone posttranslational modification
  • Our xMAP™ instruments (FlexMAP 3D and Luminex-200 systems) with high-throughput fluidics are suspension array systems capable of analyzing hundreds of biomolecules in a single sample (> 10 μL in 96 and 384-well formats)

For more information about different Luminex assay panels available, please contact [email protected] or build customized Luminex assay using these links:  ProcartaPlex, MilliplexBio-plex, and R&D Systems. Our scientists can also develop Luminex assays for special biomarkers. 

Our Luminex assays are widely used in understanding the biology of diseases

  • Acute phase response
  • Allergy / allergen testing
  • Angiogenesis
  • Antibody isotyping
  • Apoptosis
  • Autoimmunity and rheumatology
  • Cancer growth and proliferation
  • Cancer metastasis
  • Cardiovascular disease (CVD biomarkers)
  • Cell survival
  • Cellular signaling
  • Circulating cancer biomarkers
  • COVID-19 and infection
  • Diabetes and obesity
  • Endocrine system (pituitary, thyroid, & IGFs)
  • Gene expression profiling
  • Histone H3 PTMs
  • Immune checkpoint blockade
  • Immunity
  • Inflammation
  • Kidney toxicity
  • Liver injury
  • Metabolism
  • MicroRNA profiling
  • Neurodegenerative diseases
  • Protein phosphorylation
  • Stem cell differentiation & characterization
  • Transcription factors-specific DNA binding
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We Simultaneously Measure 80 Human Cytokines from Each Sample at Protein and mRNA Levels

table of various analytes that can be analyzed using luminex platform

Example Data

Bar graph showing cytokine levels PBMC isolateed from psoriatic and normal donors