Ultra-Sensitive Digital Immunoassay (SIMOA)

  • A fully automated ultrasensitive immunoassay or digital ELISA based upon the isolation of individual immunocomplexes on paramagnetic beads using standard ELISA reagents.
  • The main difference between SIMOA and traditional immunoassays lies in the ability to trap single molecules in femtoliter-sized wells, allowing for a "digital" readout of each bead to determine whether or not it is bound to the target analyte.
  • Ability to detect and quantify low-abundance biomarkers difficult or impossible to measure with other conventional assays.
  • Quantification of protein level as low as 1 fg/mL in the samples. The digital nature of the SIMOA technology allows an average 1000X sensitivity increase over conventional ELISA assays with CVs <10%.